Sweetpotato infected by Soft-rot, Rhizopus nigricans, has been investigated for its biochemical changes. The results of the present experiment are summarized as follows:
1. From the Soft-rotted tuber, nineteen Ehrlich´s positive substances and three fluorescent compounds which had not been found in a healthy tuber were detected with thin layer chromatography.
2, Ipomeamarone and umbelliferone, each of which are predominating substances among the Ehrlich´s positive and fluorescent compounds, were isolated by silica gel column chromatography and studied for their identities.
3. Time course biosynthesis of the metabolites was studied and discussed for their possible biosynthetic pathways.
4. Among the metabolites, ipomeamarone and ipomeamarone-like substances have enzyme: inhibitive action against α-amylase and probably other enzymes; the inhibition type was determined as the uncompetitive one.
5. Ipomeamarone obtained from soft-rotted sweetpotato was proved to have an uncoupling action as 2, 4-dinitrophenol.
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